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Recombinant Protein A Sepharose FF

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Recombinant Protein A Sepharose FF

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Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd

Verified Supplier


Business type: Manufacturer, Exporter

Telephone : 86-571-82872376

Room 11-08, HuaRui Building, No.66, Jianshe Road 1st, Xiaoshan,Hangzhou, Zhejiang, China 311202

Product Details:

Place of Origin: Hangzhou
Brand Name: NUPTEC
Certification: ISO9001:2008
Model Number: NRPA06L

Payment & Shipping Terms:

Minimum Order Quantity: 50ml
Price: Negotiable
Packaging Details: Bottle packing
Delivery Time: Depand on stock, without stock need 4 weeks for produce.
Payment Terms: 100% T/T, Paypal
Supply Ability: 10L/month
Detailed Product Description


Recombinant  Protein A Sepharose FF , Protein A Sepharose binding reagent

  • Product Name: Alkali-tolerant Protein A agarose FF
  • Catalog Number:NRPB06L, NRPB06S (prepacked column)
  • Packing details: 10 ml, 100 ml, 1L, 1 ml(prepacked column), 2 ml(prepacked column), 5ml(prepacked column), 10ml(prepacked column)
  • Storage condition: 4oC
  • Company Group:                                                                                                                      Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd. (NUPTEC----Sales Department)

    Add:   Room 11-08, HuaRui Building, No.66, Jianshe Road 1st, Xiaoshan,

              Hangzhou, Zhejiang, China 311202

    Hangzhou NUPTEC Rising Bioproducts Incorporation, Ltd. ( NUPTEC----GMP Manufactory)

    Add:   600 21st Boulevard, Xiasha Economy and Technology District,

              Hangzhou, China 310018


Protein A is a cell wall protein of Staphylococcus aureus and contains five functional domains that can bind to the Fc fragment of IgG. Structural modification of nature protein A improved its alkaline resistance. Therefore, NaOH could be used for removal of pyrogen and cleaning. The advantages of a spacer arm of agarose combined with the directed binding sites of recombinant protein A ensure the oriented coupling between agarose and recombinant protein A as well as the high affinity of recombinant protein A with substances.


Column characteristics:

Support 6% highly cross-linked spherical agarose
Ligand rat-PA
Ligand Density 5~6 mg/ml
Particle Size 45~165 μm
Maximum Flow Rate 700 cm/h
Recommended Linear Flow Rate 20~100 cm/h
pH Limits pH 3~13
Maximum Operating pressure 0.3 MPa
Capacity ≥50 mg/ml rabbit IgG or ≥50 mg/ml human IgG
Storage 4 oC to 8 oC in 20% ethanol


Performing a separation:

Binding buffer: 20 mM sodium phosphate, 150 mM NaCl, pH 7.4

Elution buffer: 100 mM sodium citrate, pH 4.0

Neutralization buffer: 1M Tris-HCl, pH 9.0

1) Wash the prepacked 1 ml column with 5~10 column volumes of distilled water to remove 20% ethanol.

2) Equilibrate the column with 5~10 column volumes of binding buffer.

3) Dilute 5 ml rabbit serum with binding buffer to 50 ml. Filtrate the diluted serum through a 0.45 レm filter and load the sample.

4) Wash with 10 column volumes of binding buffer.

5) Elute with 5 column volumes of elution buffer and neutralize collect fractions with neutralization buffer.

6) After each separation cycle, regenerate the resin by washing with approximately 3~5 column volumes of 0.1 M NaOH(pH 3.0).

7)Confirm the purity of the collected antibody by SDS-PAGE analysis (Figure 1, >95% purity).

                                                                Recombinant Protein A Sepharose FF

Figure 1

M- Protein Molecular Weight Marker

1-Rabbit Antibody (non-reduced)

2-Rabbit antibody (reduced)


1)     It is easy and simple to use the prepacked column. It is not necessary to need any equipment to purify antibody.

2)     Be sure that the antibody is stable under the elution conditions selected. If there is any doubt, titrate the antibody fraction to neutrality immediately upon elution in order not to lose biological activity. To accomplish this, prefill the fraction vessels to 5~10% of the intended fraction volume with a buffer concentrate at about pH7.5 (for example 1 M Tris-HCl or 1 M sodium phosphate).

3)     Make sure temperature of the equilibration buffer identical to that of the packed column to avoid air bubbles. If air has entered the column, the column should be repacked.

4)     It is recommended to regenerate the resin once after 5 times separation cycle in order to extend the service life.

5)     After 10 times separation cycle, wash the resin with 5 column volumes of 0.1~0.5M NaOH and 5~10 column volumes of 70% ethanol sequentially to remove hydrophobic substances.

6)     For longer periods of storage, keep at 4~8oC in 20% ethanol.





  • Time:December 13-15, 2012           1st Shenzhen International Biotech Innovation Forum & Exhibition
  • Time: March 13-15, 2013                 PCHI2013 Personal Care and Homecare Ingredients

  • Time: March 18-20, 2013                 BIT’s 5th Annual International Congress of Antibodies-2013

  • Time: March 22-24, 2013                 The 18th Shanghai International Beauty, Hairdressing & Cosmetics Expo

  • Time: March 30- April 1st , 2013        The 25th Zhengzhou International Beauty & Cosmetics Expo-2013

  • Time: April 14-16, 2013                    China (Wu Han) International Beauty and Cosmetic Products Exhibition

  • Time: April 18-21, 2013                    The 29th (Spring) Chengdu beauty & cosmetics Exhibition-2013

  • Time: Februry 19-21, 2014                PCHI2014 Personal Care and Homecare Ingredients

  • Time: April 25-28, 2014                     BIT’s 6th Annual International Congress of Antibodies-2014

Recombinant Protein A Sepharose FF

Recombinant Protein A Sepharose FF





GMP Manufactory :

We have 2,000 square meters of manufacturing base including 600 square meters GMP facility with class 100,000 US FED-STD-209E clean room standards Company and with certification of ISO9001:2008.


Recombinant Protein A Sepharose FF

Recombinant Protein A Sepharose FF





GMP Manufactory ISO9001:2008 Certification: 



Recombinant Protein A Sepharose FF


Recombinant Protein A Sepharose FF


Certification of safe transport by air :

Recombinant Protein A Sepharose FF

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