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Recombinant Streptococcus Bio-separation protein G Sepharose FF

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Recombinant Streptococcus Bio-separation protein G Sepharose FF

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Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd

Verified Supplier

Hangzhou

Business type: Manufacturer, Exporter

Telephone : 86-571-82872376

Room 11-08, HuaRui Building, No.66, Jianshe Road 1st, Xiaoshan,Hangzhou, Zhejiang, China 311202

Product Details:

Place of Origin: Hangzhou
Brand Name: NUPTEC
Certification: ISO9001:2008
Model Number: NRPA02L

Payment & Shipping Terms:

Minimum Order Quantity: 50ml
Price: Negotiable
Packaging Details: Bottle packing
Delivery Time: Depand on stock, without stock need 4 weeks for produce.
Payment Terms: 100% T/T, Paypal
Supply Ability: 10L/month
Detailed Product Description

 

Recombinant Streptococcus protein G Sepharose FF for Protein G agarose binding reagent , purification , Bio-separation

  • Product Name:   Recombinant Protein G Conjugated to agarose Resin
  • Catalog Number:  NRPB02L, NRPB02S (prepacked column)
  • Packing details:   10 ml, 100 ml, 1L, 1 ml(prepacked column), 2 ml(prepacked column), 5ml(prepacked column), 10ml(prepacked column)
  • Storage condition:   4oC
  • Company Group:                                                                                                                      Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd. (NUPTEC----Sales Department)

    Add:   Room 11-08, HuaRui Building, No.66, Jianshe Road 1st, Xiaoshan,

              Hangzhou, Zhejiang, China 311202

    Hangzhou NUPTEC Rising Bioproducts Incorporation, Ltd. ( NUPTEC----GMP Manufactory)

    Add:   600 21st Boulevard, Xiasha Economy and Technology District,

              Hangzhou, China 310018

Introduction:

Protein G is a bacterial cell wall protein expressing at the cell surface of some group C and group G Streptococcal strains. Protein G binds specifically to Fc regions of many mammalian immunoglobulins and is commonly used as affinity adsorbent to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites, tissue culture supernatants and other biological fluids.

 

Column characteristics:

 

Support 6% highly cross-linked spherical agarose
Ligand Recombinant protein G
Ligand Density 4~5 mg/ml
Particle Size 45~165 μm
Maximum Flow Rate 300 cm/h
Recommended Linear Flow Rate 50~150 cm/h
pH Limits pH 3~9
Maximum Operating pressure 0.3 MPa
Capacity ≥50mg/ml rabbit IgG or ≥50mg/ml human IgG
Storage 4 oC to 8 oC in 20% ethanol

 

Some typical binding and elution conditions with proteinG agarose:

 

Species Antibody Class Protein G binding pH value ofbinding buffer pH value of elution buffer
Human IgG1 +++ 6.0~7.0 3.5~4.5
IgG2 +++ 6.0~7.0 3.5~4.5
IgG3 +++ 8.0~9.0 ≤7.0
IgG4 +++ 7.0~8.0 2.5~4.5
Cow IgG2 +++ 7.0~8.0 2.0
Goat IgG2 +++ 7.0~8.0 5.8
Mouse IgG1 ++ 8.0~9.0 5.5~7.5
IgG2a +++ 7.0~8.0 4.5~5.5
IgG2b +++ 7.0 3.5~4.5
IgG3 +++ 7.0 4.0~7.0
Rat IgG1 + ≥9.0 7.0~8.0
IgG2a +++ ≥9.0 ≤8.0
IgG2b + ≥9.0 ≤8.0
IgG2c ++ 8.0~9.0 3.0~4.0
Rabbit IgG +++ 7.4 2.7~4.0

“+” represents the binding strength

 

Performing a separation:

Binding buffer: 20 mM phosphate buffered saline, pH 7.4

Elution buffer: 100 mM glycine-HCl buffer, pH 2.7

Neutralization buffer: 1M Tris-HCl, pH 9.0

1)      Wash the prepacked 1 ml column with 5~10 column volumes of distilled water to remove 20% ethanol.

2)      Equilibrate the column with 5~10 column volumes of binding buffer.

3)      Dilute 5 ml rabbit serum with binding buffer to 50 ml. Filtrate the diluted serum through a 0.45 レm filter and load the sample.

4)      Wash with 5~10 column volumes of binding buffer.

5)      Elute with 5 column volumes of elution buffer and neutralize collect fractions with neutralization buffer.

6)      After each separation cycle, regenerate the resin by washing with approximately 3~5 column volumes of 0.1 M citrate buffer (pH 2.7).

7)       Confirm the purity of the collected antibody by SDS-PAGE analysis (Figure 1, >95% purity).

 

                                                                         Recombinant Streptococcus Bio-separation protein G Sepharose FF

Notice:

1)         It is easy and simple to use the prepacked column. It is not necessary to need any equipment to purify antibody.

2)         Be sure that the antibody is stable under the elution conditions selected. If there is any doubt, titrate the antibody fraction to neutrality immediately upon elution in order not to lose biological activity. To accomplish this, prefill the fraction vessels to 5~10% of the intended fraction volume with a buffer concentrate at about pH7.5 (for example 1 M Tris-HCl or 1 M sodium phosphate).

3)         Make sure temperature of the equilibration buffer identical to that of the packed column to avoid air bubbles. If air has entered the column, the column should be repacked.

4)         It is recommended to regenerate the resin once after 5 times separation cycle in order to extend the service life.

5)         After 10 times separation cycle, wash the resin with 5 column volumes of 20% ethanol and 5~10 column volumes of 70% ethanol sequentially to remove hydrophobic substances.

6)         For longer periods of storage, keep at 4~8oC in 20% ethanol.

 

 

 

NUPTEC EXHIBITION History: 

  • Time:December 13-15, 2012           1st Shenzhen International Biotech Innovation Forum & Exhibition
  • Time: March 13-15, 2013                 PCHI2013 Personal Care and Homecare Ingredients

  • Time: March 18-20, 2013                 BIT’s 5th Annual International Congress of Antibodies-2013

  • Time: March 22-24, 2013                 The 18th Shanghai International Beauty, Hairdressing & Cosmetics Expo

  • Time: March 30- April 1st , 2013        The 25th Zhengzhou International Beauty & Cosmetics Expo-2013

  • Time: April 14-16, 2013                    China (Wu Han) International Beauty and Cosmetic Products Exhibition

  • Time: April 18-21, 2013                    The 29th (Spring) Chengdu beauty & cosmetics Exhibition-2013

  • Time: Februry 19-21, 2014                PCHI2014 Personal Care and Homecare Ingredients

  • Time: April 25-28, 2014                     BIT’s 6th Annual International Congress of Antibodies-2014

Recombinant Streptococcus Bio-separation protein G Sepharose FF

Recombinant Streptococcus Bio-separation protein G Sepharose FF

 

 

 

 

GMP Manufactory :

We have 2,000 square meters of manufacturing base including 600 square meters GMP facility with class 100,000 US FED-STD-209E clean room standards Company and with certification of ISO9001:2008.

 

Recombinant Streptococcus Bio-separation protein G Sepharose FF

Recombinant Streptococcus Bio-separation protein G Sepharose FF

 

 

 

 

GMP Manufactory ISO9001:2008 Certification: 

 

 

Recombinant Streptococcus Bio-separation protein G Sepharose FF

 

Recombinant Streptococcus Bio-separation protein G Sepharose FF

 

Certification of safe transport by air :

Recombinant Streptococcus Bio-separation protein G Sepharose FF

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