M&F Biotech Co.,Ltd
Business type: Manufacturer,Distributor/Wholesaler,Exporter,Seller
Telephone : 86-571-28181930
Jing Yuan beiyuan ,Jianggan district ,Hanzhou ,China .
|Place of Origin:||china|
|Minimum Order Quantity:||1kit|
|Packaging Details:||48T/96T /box|
|Delivery Time:||3 days|
|Payment Terms:||TT /LC|
This kit allows for the determination of bFGF concentrations in Mouse serum, tissue, and other biological fluids.
Principle of the assay
The kit assay Mouse bFGF level in the sample,use Purified Mouse bFGF to coat microtiter plate wells, make solid-phase antibody, then add bFGF to wells, Combined bFGF antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of bFGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.